ViroMed obtains these primary cells from 7- to 15-day old New Zealand white rabbit kidneys that have been treated with enzymes. After centrifuging the cell suspension and discarding the enzyme preparation, the cells are planted in minimum essential medium (Eagle), with 10% heat-inactivated fetal bovine serum. The resulting epithelial-like cells are susceptible to a variety of viruses.

Culture Medium
Minimum essential medium (Eagle), with 10% heat-inactivated fetal bovine serum.

Growth Characteristics
Cells seeded at a concentration of 1x10⁵ cells/cm² in the above culture medium will be 100% confluent in 7 days.

Morphology
Epithelial-like.

Common Utilization
Isolation of Herpes simplex Virus.