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COLLECTION AND TRANSPORT PROCEDURES

Blood

  • Collect 1 tube (4-7ml) of Heparinized (green top) or EDTA (lavender top) blood.
  • Ship overnight with 1-2 cold packs, except blood for HIV culture, which should be kept at room temperature.

Bone marrow
  • Collect 2 ml in EDTA or heparin.
  • Ship overnight with 1-2 cold packs.

Bronchial wash/brush or alveolar lavages
  • Collect in a sterile container.
  • Ship overnight with 1-2 cold packs.

CSF
  • Collect 1 ml, if possible, in a sterile container.
  • Ship overnight with 1-2 cold packs.

Dermal -
Lesions / Vesicles
  • Collect vesicle fluid with swab and, using same swab, collect cells from base of lesion. (For non-vesicular, use swab to collect cells from base of lesion.) Place swab in M4. Or collect with viral culturette.
  • Ship overnight with 1-2 cold packs.

Eye swab or scrapings
  • Place swabs or scrapings in ViroMed transport media or collect on a viral culturette.
  • Ship overnight with 1-2 cold packs.

Fluid
  • Collect 1ml in a sterile container.
  • Ship overnight with 1-2 cold packs.

Nasopharyngeal
  • Collect 2 NP swabs. Place both swabs in Viral, or collect on a viral culturette.
  • Ship overnight with 1-2 cold packs.

Sputum
  • Collect in a sterile container.
  • Ship overnight with 1-2 cold packs.

Stool
  • Collect 1 gram in a clean container.
  • Ship overnight with 1-2 cold packs.

Swab of any infected site
  • Collect on a sterile swab and place in Viral.
  • Ship overnight with 1-2 cold packs.

Cervical brush biopsy for Human Papillomavirus (HPV)
  • Scraping of infected site with Digene Cervical Sampler Brush.
  • Biopsy placed immediately into Digene Specimen Transport Medium.
  • Ship scrapings/brushings at room temperature overnight. Ship biopsy frozen on dry ice overnight.

Throat
  • Collect with sterile swab and place in Viral transport media, or collect on a viral culturette.
  • Ship overnight with 1-2 cold packs.

Tissue
  • Place in Viral transport media immediately after collection.
  • Ship overnight with 1-2 cold packs.

Urine
    Adult: Collect 5 ml in sterile container.
    Infant: Collect, if possible, 1 ml in sterile container.
  • Ship overnight with 1-2 cold packs.

Serum
  • Collect blood in a red top tube or serum separator tube (SST). Allow blood to clot for 30 minutes at room temperature. Centrifuge, transfer serum to a plastic vial and refrigerate.
  • Ship overnight with 1-2 cold packs.

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Specimen Guide